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1.1 This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.
| Author | ASTM |
|---|---|
| Editor | ASTM |
| Document type | Standard |
| Format | File |
| ICS | 17.180.30 : Optical measuring instruments |
| Number of pages | 8 |
| Replace | ASTM E3143-18a + Redline |
| Set | ASTMVOL1402 |
| Year | 2018 |
| Document history | ASTM E3143-18a + Redline |
| Country | USA |
| Keyword | ASTM 3143;ASTM E3143;ASTM E3143;10.1520/E3143-18B |