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ASTM E3143-18b + Redline

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ASTM E3143-18b + Redline

Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes

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1.1 This practice covers procedures for vitrifying and recording images of a suspension of liposomes with a cryo-transmission electron microscope (cryo-TEM) for the purpose of evaluating their shape, size distribution and lamellarity for quality assessment. The sample is vitrified in liquid ethane onto specially prepared holey, ultra-thin, or continuous carbon TEM grids, and imaged in a cryo-holder placed in a cryo-TEM.

Author ASTM
Editor ASTM
Document type Standard
Format File
ICS 17.180.30 : Optical measuring instruments
Number of pages 8
Replace ASTM E3143-18a + Redline
Set ASTMVOL1402
Year 2018
Document history ASTM E3143-18a + Redline
Country USA
Keyword ASTM 3143;ASTM E3143;ASTM E3143;10.1520/E3143-18B